Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis.

نویسندگان

  • Dannon J Stigers
  • Zachary I Watts
  • James E Hennessy
  • Hye-Kyung Kim
  • Romeo Martini
  • Matthew C Taylor
  • Kiyoshi Ozawa
  • Jeffrey W Keillor
  • Nicholas E Dixon
  • Christopher J Easton
چکیده

3-Chloro-Abu and 4-chloro-Nva are biosynthetically incorporated into E. coli peptidyl-Pro cis-trans isomerase B, as substitutes for Val and Leu, respectively. The extent of incorporation is up to ~90%, and substituted protein is catalytically active. By contrast, 4-chloro-Val is not an effective replacement for Ile.

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عنوان ژورنال:
  • Chemical communications

دوره 47 6  شماره 

صفحات  -

تاریخ انتشار 2011